The course covers theory and advanced image analysis techniques in transmission electron microscopy (TEM). In the lecture the following topics are explained:

  • basic anatomy of a TEM, electron guns, holders and detectors, energy filters, vacuum system
  • theory of image formation, how electrons interact with material, amplitude and phase contrast, contrast transfer function
  • sample preparation and image acquisition in single particle TEM and tomography
  • 2D and 3D image analysis in single particle TEM and tomography
  • 2D crystallography
  • refinement and validation of 3D reconstructions
  • visualisation and interpretation of TEM results

During the seminar you will analyse current TEM results from subject-specific English literature, summarize the key aspects of the work and discuss the research outcome critically.